Based on the theoretical study of the dry friction isolation system under the random vibration, a formula for the description of the acceleration response of metal rubber (MR) isolation system under random vibration was derived. An experimental research for the MR isolator subject to random vibration excitation was carried out, and the effects of the relative density, the preliminary deformation, and the acceleration level of excitation on the root mean square of acceleration response for the isolation system were analyzed and depicted in curves. The theoretical root mean square of acceleration response was compared with the experimental ones, and the range for the practical use was determined.