Superconducting quantum interference device (SQUID) amplifier is best known for its low input impedance, low noise and low power consumption. Nowadays it is widely used for detecting the weak signals. Compared with other methods, the Nb/Al-AlOx/Nb structure Josephson junction based SQUID has the advantages of high transition temperature, high voltage flux modulation index and good heat recycle ability, wide critical voltage range, so it is a very good option for making SQUID amplifier. In this work, we fabricate the overdamped Josephson junction and washer dc SQUID, and test the I-V characteristics at He3 3 K stage temperature and calculate the current resolution of SQUID. The result of SQUID modulation property is good. The magnification becomes larger after increasing the input line number of loops, and the system noise becomes smaller after the join of the LC filter. This work is very important for designing and manufacturing transition edge sensor readout circuits.