The surface alignment of liquid crystals is very important in preparing liquid crystal cells. The orientational effect of liquid crystal and polymide molecules on Langmuir-Blodgett(LB) films has been studied. The degree of orientation of the polyimide chain in LB films is weaker than that in strong rubbing films. The orientational effect of liquid crystals on the polyimide LB films is worse than that on the strong rubbing films. The surface azimuthal anchoring energy of liquid crystals on the polyimide LB films was measured. The intermolecular interaction between liquid crystal and polymer molecules is considered to be very important for the alignment of liquid crystals on the polymer surface.