The traditional measurements for environmental noise exposure-dose are inapplicable to the sound samples with fixed playing duration used in subjective evaluation experiment. In this paper, we propose a method for exposure-dose measurement of sound samples with fixed duration. The method based on the short-term exposure-dose values during the playing time of the sound samples. An entire analysis on the potential influencing factors are carried out, including the short-term analysis period, the statistical properties of the short-term exposure-dose values and four different frequency weightings. Then 11 indices are used to measure the exposure-dose of the fixed duration sound sample, which lays the groundwork for establishing the dose-annoyance relationships via subjective evaluating experiments.