Measuring the temperature in vacuum environment is more complex than in atmospheric environment. For example, high vacuum will cause the thermocouple sensor surface desorption, and the mechanism of heat transfer is also different. Therefore, there are some uncertainties if the thermocouple is used to measure the gas temperature in vacuum condition. In the present paper, tunable diode laser absorption spectroscopy (TDLAS) is employed to measure the gas temperature and also explore the application prospect of TDLAS temperature measurement technology in vacuum environment. During the thermal vacuum experiments, the vacuum gas cell is immersed in the thermostatic bath, and the gas temperature is determined by TDLAS. Meanwhile, a standard Pt-resistance is used to measure the thermostatic bath temperature. The results show that the temperatures of the gas and thermostatic bath are highly consistent with each other, and the difference between the two temperatures is less than 0.2 ℃ when the thermostatic bath is stable.