Since the third generation synchrotron radiation source came into service, the X-ray techniques which relate to coherent property have quickly developed and been widely used. Typically, X-ray phase contrast imaging has become a conventional imaging method. The X-ray techniques, such as coherence scattering, coherent diffraction imaging, and photon correlation spectroscopy, have received more attention and shown unique superiority in the field of high spatial and time resolution. So quantifying the coherent property of X-ray source is meaningful for those novel X-ray techniques. In this article, based on the Talbot self-imaging phenomenon, the spatial coherent property and the scale of X-ray source of X-ray imaging and biomedical application beam line in Shanghai synchrotron radiation facility are measured. The results show that when the photon energy is 33.2 keV, the spatial coherence length is 8.84 μm and source size is 23 μm in the vertical direction, and the test result is in agreement with the theoretical value.